The creatinine clearance test compares the level of creatinine in urine with the creatinine level in the blood. It is an imperfect measure of glomerular filtration rate. The use of creatinine to estimate glomerular filtration rate is based upon three assumptions: (1) creatinine is filtered through the glomerulus, (2) relatively low amounts of creatinine are reabsorbed through the nephron tubule, and (3) creatinine production is constant overtime. Although creatinine can provide a rough estimate of glomerular filtration rate, error may be encountered. Such factors as increased tubular reabsorption of creatinine, reduced creatinine generation from muscle tissue, and dietary changes in nitrogenous compounds may affect creatinine clearance.
Showing posts with label Clinical Chemistry. Show all posts
Showing posts with label Clinical Chemistry. Show all posts
Different Enzymatic Methods for Glucose
Glucose is measured in whole blood, plasma, serum or cerebrospinal fluid (CSF). Historically, blood glucose values were given in terms of whole blood, but most laboratories now measure and report plasma or serum glucose levels. Because red blood cells (erythrocytes) have a higher concentration of protein (e.g., hemoglobin) than serum, serum has a higher water content and consequently more dissolved glucose than does whole blood. Current procedures that measure glucose use enzymatic methodology.
C- Reactive Protein Latex Agglutination Test: Principle, Procedure and Result
Principle:
Latex particles coated with antibody to CRP are reacted with patient serum. In this case, the CRP is acting as the antigen. If CRP is present above normal threshold levels, the antigen–antibody combination will result in a visible agglutination reaction. An elevated CRP level is a sensitive, although nonspecific, indicator of inflammation.
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