Principle:
Pathogen detection by the PCR is based on the amplification of specific regions of the pathogen genome. The amplified product is detected via fluorescent dyes. These are usually linked to oligonucleotide probes which bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run allows the detection and quantitation of the accumulating product without having to re-open the reaction tubes after the PCR run.
RNA Extraction Procedure:(See DNA Extraction using QIAmp DSP Virus Kit DNA/RNA Extraction Procedure)
Master Mix Preparation(pre-PCR):
- Please use the following pipetting scheme:
number of samples
|
1
| |
HIV-RG Master A
|
12 ul
| |
Prep. of
|
HCV-RG MasterB
|
18 ul
|
Mastermix
|
HCV-RG IC
|
2 ul
|
Total volume
|
32 ul
| |
Prep of
|
Mastermix
|
30 ul
|
PCR assay
|
Sample
|
20 ul
|
Total volume
|
50 ul
|
- Mix the solution by pipetting up and down. Close the PCR tubes and ready to load the tubes in Rotorgene 6000 for amplification.
Interpretation of Results:
- A signal is detected in fluorescence channel Cycling A.FAM.
- In fluorescence channel Cycling A.FAM no signal is detected. At the same time, a signal from the internal control appears in the Cycling A.ROX channel.
- No signal is detected in the Cycling Green or Cycling A.FAM channels, or in the Cycling Orange or the Cycling A.ROX channels.
Reference Values:
- HIV- RNA >72 IU/ml: The result is within the determined test range.
HERPES VIRUS PERMANENT TREATMENT The herpes simplex virus is a contagious virus that can be passed from person to person through direct contact.Children will often contract HSV-1 from early contact with an infected adult. They then carry the virus with them for the rest of their life.Infection with HSV-1 can happen from general interactions such as eating from the same utensils, sharing lip balm, or kissing. The virus spreads more quickly when an infected person is experiencing an outbreak. Additionally,it is possible to get genital herpes from HSV-1 if the individual has had cold sores and performed sexual activities during that time.HSV-2 is contracted through forms of sexual contact with a person who has HSV-2. It is estimated that around 20 percent of sexually active adults within the United States have been infected with HSV-2, according to the American Academy of Dermatology (AAD). ( AAD ) While HSV-2 infections are spread by coming into contact with a herpes sore, the AAD reports that most people get HSV-1 from an infected person who is a symptomatic, or does not have sores.having multiple sex partners being female having another sexually transmitted infection (STI) having a weakened immune system
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