Vibrio Cholerae Agglutinating Sera (MUREX) (Slide Agglutination Test)

Vibrio Cholerae Agglutinating Sera test whether a culture is positive for the O-antigen of the lipopolysaccharides of Vibrio cholerae 01 that exist in two forms termed Inaba and Ogawa.

Procedure:

For 2 minutes

• Put separate drops (40µl each) of saline on a glass slide 
• Pick up colonies and emulsify with a drop of saline to give a smooth, fairly dense suspension 

For 1 minute

• To one suspension, use as a control, add 1 drop (40µl) of saline and mix. 
• To the other suspension, add 1 drop (40µl) of antiserum and mix. 
• Rock the slide for 1 minute and observe for agglutination