Principle:
This is a test to detect the presence of Cryptococcus neoformans in a specimen.Negative staining produces a black, semi-opaque background that makes the clear capsule more visible.
Procedure:
This is a test to detect the presence of Cryptococcus neoformans in a specimen.Negative staining produces a black, semi-opaque background that makes the clear capsule more visible.
Procedure:
- Mix one drop of specimen (CSF) with one drop of India Ink on a clean slide.
- Cover with cover slip.
- Examine under LPO and switch to HPO.
- If preparation is too dark, it may be diluted with a small drop of H2O.
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