Principle:
KOH Preparation of clinical material is used to facilitate the observation of fungal forms. The potassium hydroxide lyses or destroys most cellular material /debris and allows the yeast, fungus, cells, or components to be more easily seen.
KOH Preparation of clinical material is used to facilitate the observation of fungal forms. The potassium hydroxide lyses or destroys most cellular material /debris and allows the yeast, fungus, cells, or components to be more easily seen.
Procedure:
- Mix specimen with 10% - 20% KOH solution on a clean slide.
- Cover with a cover slip and press gently to make thin mount.
- Utilize gentle heating to aid clearing.
- Scan under the low power field (LPO) with reduced lighting.
- Switch to high power to scan or check the presence of suspected fungal element.
- The presence of yeast, budding yeast, pseudohyphae, septate hyphae or chlamydospores is a positive result.
- The absence of fungal elements is a negative result.
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